CAP Pro Course - Phlebotomy - Common Pitfalls in Specimen Processing

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Author: Carol Becker, MS, MLS(ASCP)CM
Reviewer: Alexandru Casapu, MBA, MLS(ASCP)CM, PBTCM

Continuing Education Credits

Objectives

  • Recognize the importance of certain preanalytical variables in the provision of quality specimens for laboratory testing.
  • Describe specific processing and handling errors that can potentially affect laboratory testing.
  • Define common specimen rejection criteria.
  • Outline proper centrifuging procedures, including work practices that promote safe specimen processing and handling.

Course Outline

  • Recognize the importance of certain preanalytical variables in the provision of quality specimens for laboratory testing.
      • Preanalytical Variables
      • Common Sources of Preanalytical Error in Blood Collection
      • Blood Collection Additives and Their Use
      • Primary Specimen Identification and Labeling
      • Guidelines for Body Fluid Labeling and Handling
      • General Considerations in Handling Common Body Fluids
      • Which of the following steps is the most important in the process of specimen collection by venipuncture or dermal puncture?
      • Select the process below that is not a part of the preanalytical phase of the laboratory testing.
      • Jolene, a new specimen processor, receives a purple-top EDTA tube of blood and a requisition for prothrombin time (PT) and activated partial thrombopl...
      • Which of the following blood collection tube types is appropriate for collecting a hematology test that requires a whole blood EDTA sample?
  • Describe specific processing and handling errors that can potentially affect laboratory testing.
      • Common Specimen Processing and Handling Errors
      • Effects of Transportation, Time, and Temperature on Blood Specimens
      • Processing Serum and Plasma Specimens
      • A Note About Specimen Storage
      • Which of the following actions can produce hemolysis in a blood specimen?
      • An Emergency Department physician calls the laboratory to add calcitonin testing to a serum specimen collected earlier the same day. The collection de...
      • A specimen processing employee removes a gold-top tube from the centrifuge and notices there are still red blood cells in the liquid portion (serum) o...
      • Which processing error is likely to cause serum or plasma that is still found in contact with blood cells below the gel barrier to move into the cell-...
  • Define common specimen rejection criteria.
      • Common Reasons for Specimen Rejection
      • Specimen Label Correction
      • Angie receives a light green-top, lithium heparin tube from the floor for hematology testing that includes a CBC with differential and sedimentation r...
      • Several specimen issues are presented below that could warrant rejection of the specimen and possible recollection. Which one of these situations pres...
      • Based on typical laboratory specimen rejection criteria, which of the following patient samples would be acceptable for testing?
      • What is the maximum time a blood tube collected for a routine potassium test should be held prior to centrifugation?
  • Outline proper centrifuging procedures, including work practices that promote safe specimen processing and handling.
      • Phases of Centrifugation
      • Proper Centrifugation Operation
      • Disinfection Procedures for Centrifuge Spills
      • Engineering Controls and Personal Protective Equipment (PPE)
      • Serum poured into an aliquot tube is observed to have an obvious fibrin clot in it when it reaches the chemistry department for testing. What is the m...
      • Chris is a long-time employee in the specimen processing department. He occasionally opens the centrifuge as it slows to stop the rotor with his hand ...
      • Numerous, assorted blood collection tubes have just been delivered through the pneumatic tube system from several locations throughout the hospital. T...
      • What personal protective equipment (PPE) is not typically worn when processing specimens that may produce splashing?
  • References
      • References

Additional Information

Level of Instruction: Basic
Intended Audience: This program is designed as an educational and training tool for MLS, MT, and MLT personnel, phlebotomists, medical laboratory science students and interns, pathology residents, and practicing pathologists.
Author Information: Carol Becker, MS, MLS(ASCP)CM is a retired Program Director for the Clinical Laboratory Science program and Histology program at OSF St. Francis Medical Center located in Peoria, Illinois. Carol received her Master's degree at the University of St. Francis in Joliet, Illinois, and her Bachelor's degree in Medical Laboratory Science from the University of Illinois in Champaign, IL.
Reviewer Information: Alexandru Casapu, MBA, MLS(ASCP)CM, PBTCM, has over 20 years of experience as a medical laboratory scientist, section supervisor, and laboratory manager. He is the former Director of Clinical Laboratory Technology Program at Georgia Piedmont Technical College. He is currently a Program Director at MediaLab, Inc. Alex holds BS degrees in Biology and Medical Technology from Clark Atlanta University and an MBA from the University of Georgia.

This course is part of the CAP Competency Assessment Hub.

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