Case Studies in Pediatric Hematology

Continuing Education Credits

• P.A.C.E.® Contact Hours: 1 hour(s)
• Florida Board of Clinical Laboratory Science CE Credit Hours -
  General (Hematology): 1 hour(s)

Objectives

• Calculate mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and corrected white blood cell (WBC) count.
• Predict peripheral smear microscopic characteristics using basic hemogram parameters.
• Identify laboratory testing panels that best assess for hematological conditions.
• Categorize cytochemistry stains and immunophenotyping results in the setting of acute leukemias.
• Identify characteristics of macrocytic anemias.
• Differentiate between alpha and beta thalassemia.

Course Outline

• Approaching “Case Studies in Pediatric Hematology”
• Approaching Case Studies in Pediatric Hematology
• Case 1: Four-Week-Old Infant Born Prematurely
• History of Present Illness (HPI) for Candace
• Relevant Laboratory Studies for Candace
• How would you describe the image of Candace’s peripheral blood smear?
• Which hormone is responsible for the regulation of erythrocyte production?
• Patient Diagnosis: Anemia of Prematurity
• Review of Hematopoiesis
• Review of Hematopoiesis, continued
• Match the listed peripheral blood cells with their correct progenitor cell lineage, either myeloid or lymphoid.
• Case 2: Eight-Month-Old Born in Mexico
• History of Present Illness (HPI) for Nicolás
• Which laboratory assay represents the amount of iron stored in the body?
• Relevant Laboratory Studies for Nicholás
• How would you describe the representative image of Nicolás' peripheral blood smear?
• Patient Diagnosis: Iron Deficiency Anemia
• True or False: In iron deficiency anemia, as serum iron decreases total iron binding capacity (TIBC) increases.
• Comparing Iron Studies in Iron Deficiency Anemia versus Anemia of Chronic Disease
• Case 3: Five-Year-Old with Stomach Pain
• History of Present Illness (HPI) for Patrick
• Relevant Laboratory Studies for Patrick
• How would you describe the representative image of Patrick’s peripheral blood smear?
• Identify the composition of the RBC inclusions seen in this image.
• Patient Diagnosis: Lead Poisoning
• Blood Lead Level Collection and Processing Issues
• Which of the following is not appropriate for blood lead level collection and processing?
• Case 4: 13-Year-Old with Weight Loss
• History of Present Illness (HPI) for Danielle
• Relevant Laboratory Studies for Danielle
• How would you describe the representative image of Danielle’s peripheral blood smear?
• Which white blood cell abnormality would you expect to see on the peripheral blood smear of this patient?
• Patient Diagnosis: Megaloblastic Anemia
• True or False: Megaloblastic anemias result in abnormalities of cytoplasmic maturation.
• Folic Acid versus Vitamin B12 (Cobalamin) Deficiency
• Case 5: 2-Year-Old with "Flu" for a Month
• History of Present Illness (HPI) for Benton
• Relevant Laboratory Studies for Benton
• How would you describe the representative image of Benton’s peripheral blood smear?
• True or False: Staining for myeloperoxidase (MPO) is significant in distinguishing myeloid blasts from lymphoid blasts as MPO is present in granules o...
• Patient Diagnosis: Acute Lymphoblastic/Lymphocytic Leukemia (ALL)
• Review of Immunophenotyping and Cytochemistry Staining in Leukemias
• Case 6: 12-Month-Old with Dark Urine
• History of Present Illness (HPI) for Theo
• Relevant Laboratory Studies for Theo
• How would you describe the representative images of Theo’s peripheral blood smear?
• Hemoglobin Structure
• Hemoglobin Structure, continued
• Patient Diagnosis: Beta Thalassemia Major
• Key Points of Alpha Thalassemia
• Key Points of Beta Thalassemia
• True or False: Haptoglobin increases in hemolytic processes.
• References
• References

Additional Information